Journal: BMC Bioinformatics
Article Title: Impact of the spotted microarray preprocessing method on fold-change compression and variance stability
doi: 10.1186/1471-2105-12-413
Figure Lengend Snippet: Scatter plot between microarray and Taqman . Scatter plot of log2 fold-changes obtained from quantitative PCR versus the log2 fold-changes obtained from microarray after the best- (Standard background correction with log2 transformation) and the worst- (No background correction with glog transformation) preprocessing methods. Microarray data leads to fold-change compressions for many genes, especially with the No background correction and the glog transformation (see Figure2).
Article Snippet: As our study focuses on the preprocessing of spotted microarray data, we decided to perform analyses on a high density spotted oligonucleotide microarray platform (CodeLink Human Whole Genome from GE Healthcare) as well as on a low density spotted cDNA microarray platform (Dualchip Microarray from Eppendorf).
Techniques: Microarray, Real-time Polymerase Chain Reaction, Transformation Assay